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MedChemExpress j62080 4 aminosalicylic acid pas acros organics 104620050 pretomanid pmd
J62080 4 Aminosalicylic Acid Pas Acros Organics 104620050 Pretomanid Pmd, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pretomanid+pmd/4-Aminosalicylic+acid/pm42030160-173-12-21
Average 94 stars, based on 1 article reviews

j62080 4 aminosalicylic acid pas acros organics 104620050 pretomanid pmd - by Bioz Stars, 2026-09

94/100 stars

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Article Title: Mouse models of tuberculosis uniformly featuring hypoxic necrotic lesions and poor response to chemotherapy.
Article Snippet: To determine the initial bacterial implantation in the lungs, five mice were sacrificed 1 day following aerosol exposure in each infection model. Vaccinated model: drug preparation and in vivo treatment For drug treatment studies, mice received individual anti-tuberculosis drugs adminis tered as monotherapy.To determine the initial bacterial implantation in the lungs, five mice were sacrificed 1 day following aerosol exposure in each infection model. Vaccinated model: drug preparation and in vivo treatment For drug treatment studies, mice received individual anti-tuberculosis drugs adminis tered as monotherapy.. INH, PZA, MXF, and LZD were purchased from Acros Organics; RIF was obtained from Sigma-Aldrich, and BDQ and Pretomanid (PMD) were purchased from MedChemExpress.. Drug formulations were prepared fresh weekly as follows: INH at 25 mg/kg, PZA at 150 mg/kg, and MXF at 200 mg/kg were dissolved in sterile water.Drug formulations were prepared fresh weekly as follows: INH at 25 mg/kg, PZA at 150 mg/kg, and MXF at 200 mg/kg were dissolved in sterile water.

Article Title: Mouse models of tuberculosis uniformly featuring hypoxic necrotic lesions and poor response to chemotherapy
Article Snippet: For drug treatment studies, mice received individual anti-tuberculosis drugs administered as monotherapy.For drug treatment studies, mice received individual anti-tuberculosis drugs administered as monotherapy.. INH, PZA, MXF, and LZD were purchased from Acros Organics; RIF was obtained from Sigma-Aldrich, and BDQ and Pretomanid (PMD) were purchased from MedChemExpress.. Drug formulations were prepared fresh weekly as follows: INH at 25 mg/kg, PZA at 150 mg/kg, and MXF at 200 mg/kg were dissolved in sterile water.Drug formulations were prepared fresh weekly as follows: INH at 25 mg/kg, PZA at 150 mg/kg, and MXF at 200 mg/kg were dissolved in sterile water.

Article Title: Mouse Models Uniformly Featuring Human-like Lesions Harboring Drug-tolerant Mycobacterium tuberculosis
Article Snippet: For drug treatment studies, mice received individual anti-tuberculosis drugs administered as monotherapy.For drug treatment studies, mice received individual anti-tuberculosis drugs administered as monotherapy.. Isoniazid (INH), Pyrazinamide (PZA), Moxifloxacin (MXF), and Linezolid (LZD) were purchased from Acros Organics; Rifampicin (RIF) was obtained from Sigma-Aldrich, and Bedaquiline (BDQ) and Pretomanid (PMD) were purchased from MedChemExpress.. Drug formulations were prepared fresh weekly as follows: INH at 25 mg/kg, PZA at 150 mg/kg, and MXF at 200 mg/kg were dissolved in sterile water.Drug formulations were prepared fresh weekly as follows: INH at 25 mg/kg, PZA at 150 mg/kg, and MXF at 200 mg/kg were dissolved in sterile water.

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a Bacteria were starved in PBS for 21 days and then exposed to 0.5 μg/ml isoniazid (INH), 50 μg/ml ethionamide (ETH), or were starved for 14 days and then exposed to 0.5 μg/ml and 1 μg/ml delamanid (DMD), 1 μg/ml pretomanid (PMD) or equal amount of DMSO for 7 days and cultured on agar plates for CFU enumeration. Data are means ± SEM from two independent experiments each with triplicate cultures, except for PMD data, which are from one experiment with triplicate cultures. Statistical significance was assessed by one-way ANOVA followed by Dunnett’s multiple comparison test. ** P < 0.01, *** P < 0.001, **** P < 0.0001. b CFU quantification of the indicated strains after incubation in caseum mimetic for 4 weeks followed by 1 week treatment with isoniazid or pretomanid at the indicated concentrations. The stippled lines indicate a 10-fold reduction of wild-type and Δ cinA relative to DMSO-treated cultures and were used to determine the MBC 90 for these strains. Data are means ± SD of triplicate cultures and representative of two experiments. c CFU quantification of the indicated strains after incubation with 0.5 µg/ml isoniazid, 4.5 µg/ml ethionamide, 50 μg/ml delamanid, 1 μg/ml pretomanid, and 5 μg/ml rifampicin in standard growth media. Data are means ± SEM from one or two independent experiments each with triplicate cultures. Statistical significance of the difference between wild-type and Δ cinA was assessed by two-tailed, unpaired t -test, * P < 0.05; **** P < 0.0001. d Impact of antibiotics on growth of the indicated strains. Data are means ± SEM of triplicate cultures and representative of at least two experiments. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: CinA mediates multidrug tolerance in Mycobacterium tuberculosis

doi: 10.1038/s41467-022-29832-1

Figure Lengend Snippet: a Bacteria were starved in PBS for 21 days and then exposed to 0.5 μg/ml isoniazid (INH), 50 μg/ml ethionamide (ETH), or were starved for 14 days and then exposed to 0.5 μg/ml and 1 μg/ml delamanid (DMD), 1 μg/ml pretomanid (PMD) or equal amount of DMSO for 7 days and cultured on agar plates for CFU enumeration. Data are means ± SEM from two independent experiments each with triplicate cultures, except for PMD data, which are from one experiment with triplicate cultures. Statistical significance was assessed by one-way ANOVA followed by Dunnett’s multiple comparison test. ** P < 0.01, *** P < 0.001, **** P < 0.0001. b CFU quantification of the indicated strains after incubation in caseum mimetic for 4 weeks followed by 1 week treatment with isoniazid or pretomanid at the indicated concentrations. The stippled lines indicate a 10-fold reduction of wild-type and Δ cinA relative to DMSO-treated cultures and were used to determine the MBC 90 for these strains. Data are means ± SD of triplicate cultures and representative of two experiments. c CFU quantification of the indicated strains after incubation with 0.5 µg/ml isoniazid, 4.5 µg/ml ethionamide, 50 μg/ml delamanid, 1 μg/ml pretomanid, and 5 μg/ml rifampicin in standard growth media. Data are means ± SEM from one or two independent experiments each with triplicate cultures. Statistical significance of the difference between wild-type and Δ cinA was assessed by two-tailed, unpaired t -test, * P < 0.05; **** P < 0.0001. d Impact of antibiotics on growth of the indicated strains. Data are means ± SEM of triplicate cultures and representative of at least two experiments. Source data are provided as a Source Data file.

Article Snippet: Isoniazid (INH), Rifampicin (RIF), Ethionamide (ETH), and Pretomanid (PMD) were purchased from Sigma; Delamanid (DMD) was procured from MedKoo Biosciences.

Techniques: Cell Culture, Incubation, Two Tailed Test

a MS/MS fragmentation spectra of a chemically synthesized isoniazid-NAD adduct and corresponding metabolite observed only in isoniazid-treated Mtb with the predicted mass of the INH-NAD adduct. Structures and annotated fragments provide confirmatory evidence of mass matching to the predicted parent adduct (Δppm = 10) and fragments corresponding to the ADP and adenine moieties of the adduct. Boxed masses additionally indicate matching collision energy-dependent fragmentation products observed in both the chemical standard and INH-treated Mtb . Additional non-matching masses correspond to likely fragmentation products of co-gating parent masses that entered the collision cell with the INH-NAD adduct as observed in the 0 V spectra. b Quantification of the isoniazid-NAD adduct as a function of the isoniazid concentration that the indicated strains were exposed to. c Accumulation of isoniazid-NAD is reversed by expression of CinA with a functional pyrophosphatase domain. Data are means ± SD of six ( b ) or three ( c ) cultures. d MS/MS fragmentation spectra of a mass ion corresponding to the predicted mass of a PMD-NAD adduct observed in pretomanid-treated Mtb . Structures and annotated masses provide confirmatory evidence of mass matching to the predicted parent adduct (Δppm = 10) and fragments corresponding to the adenine, adenosine, and nicotinamide riboside-pretomanid moieties of the PMD-NAD adduct. e Quantification of the relative abundance of pretomanid-NAD adduct in the indicated strains. Data are from six independent cultures that were pooled to ensure robust detection of potential differences in adduct levels. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: CinA mediates multidrug tolerance in Mycobacterium tuberculosis

doi: 10.1038/s41467-022-29832-1

Figure Lengend Snippet: a MS/MS fragmentation spectra of a chemically synthesized isoniazid-NAD adduct and corresponding metabolite observed only in isoniazid-treated Mtb with the predicted mass of the INH-NAD adduct. Structures and annotated fragments provide confirmatory evidence of mass matching to the predicted parent adduct (Δppm = 10) and fragments corresponding to the ADP and adenine moieties of the adduct. Boxed masses additionally indicate matching collision energy-dependent fragmentation products observed in both the chemical standard and INH-treated Mtb . Additional non-matching masses correspond to likely fragmentation products of co-gating parent masses that entered the collision cell with the INH-NAD adduct as observed in the 0 V spectra. b Quantification of the isoniazid-NAD adduct as a function of the isoniazid concentration that the indicated strains were exposed to. c Accumulation of isoniazid-NAD is reversed by expression of CinA with a functional pyrophosphatase domain. Data are means ± SD of six ( b ) or three ( c ) cultures. d MS/MS fragmentation spectra of a mass ion corresponding to the predicted mass of a PMD-NAD adduct observed in pretomanid-treated Mtb . Structures and annotated masses provide confirmatory evidence of mass matching to the predicted parent adduct (Δppm = 10) and fragments corresponding to the adenine, adenosine, and nicotinamide riboside-pretomanid moieties of the PMD-NAD adduct. e Quantification of the relative abundance of pretomanid-NAD adduct in the indicated strains. Data are from six independent cultures that were pooled to ensure robust detection of potential differences in adduct levels. Source data are provided as a Source Data file.

Article Snippet: Isoniazid (INH), Rifampicin (RIF), Ethionamide (ETH), and Pretomanid (PMD) were purchased from Sigma; Delamanid (DMD) was procured from MedKoo Biosciences.

Techniques: Tandem Mass Spectroscopy, Synthesized, Concentration Assay, Expressing, Functional Assay

Balb/C mice were infected with wild-type and Δ cinA and treated with BPaL (bedaquiline 20 mg/kg/day, pretomanid 50 mg/kg/day, and linezolid 100 mg/kg/day) starting after 4 weeks of infection. CFU recovered from lungs and spleens are normalized to the beginning of BPaL treatment. Data are means ± SD of six mice. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: CinA mediates multidrug tolerance in Mycobacterium tuberculosis

doi: 10.1038/s41467-022-29832-1

Figure Lengend Snippet: Balb/C mice were infected with wild-type and Δ cinA and treated with BPaL (bedaquiline 20 mg/kg/day, pretomanid 50 mg/kg/day, and linezolid 100 mg/kg/day) starting after 4 weeks of infection. CFU recovered from lungs and spleens are normalized to the beginning of BPaL treatment. Data are means ± SD of six mice. Source data are provided as a Source Data file.

Article Snippet: Isoniazid (INH), Rifampicin (RIF), Ethionamide (ETH), and Pretomanid (PMD) were purchased from Sigma; Delamanid (DMD) was procured from MedKoo Biosciences.

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